​Berberine is an alkalid derivative found in many medicinal plants. In order to enhance the absorption of berberine, consequently to impove the ability of elimination pathogenic bacteria, the other form of berberine are interested in many research. In this study, nano berberine was produced by using rotary grinding method with Zirconium beads and its antibacterial activity agaisnt E. coli and S. aureus was investigated. Over 90% of both strains of bacteria were eliminated 24 hours after adding into nano berberine solution. The antibacteirial susceptibilioty test showed minimum inhibition concentration (MIC) of nano berberine against S. aureus was 66 µg/ml. No inhibition zone was observed with E. coli. Nano berberine is a potential option in the treatment of some diseases caused by bacteria.

Keywords: Alkaloid, berberin, bactericidal performance, absorption, nano.